General
Restoring a corrupt Library in Volocity
Restoring a corrupt Library in Volocity A Volocity library is an essential database that combines image data and metadata. If a library becomes corrupt, it may be difficult or impossible to fully restore the data without a proper backup. For this ...
Connecting a Leica DMi8 Microscope to Volocity
Connecting a Leica DMi8 Microscope to Volocity To connect a Leica DMi8 microscope to Volocity, you need to configure the microscope's Virtual COM Port (VCP). The Leica SDK, which is included with the DMi8 upon delivery, installs the necessary driver ...
Transferring Volocity Configurations Between Workstations or Users
Transferring Volocity Configurations Between Workstations or Users If you have purchased a new workstation or need to standardize settings across multiple user accounts on a single workstation, Volocity configurations can be transferred. Follow the ...
Calculating PSFs with Intermediate Magnification from Optivars
Calculating PSFs with Intermediate Magnification from Optivars In Volocity, Point Spread Functions (PSFs) are calculated in object space, also referred to as the back-projected plane or specimen plane. This approach ensures that PSFs remain ...
Playback Speed Factors for Multi-Z Plane Image Sequences
Playback Speed Factors for Multi-Z Plane Image Sequences When playing through a time-series dataset in Volocity, you may notice that playback is smoother in the XY plane mode, but significantly slower in XYZ, XZ, or YZ viewing modes. This difference ...
How to Identify Objects Inside Other Objects in Volocity
How to Identify Objects Inside Other Objects in Volocity The Internalize Objects task in Volocity allows you to identify objects that are entirely contained within other objects by assigning them a Parent ID. This feature is useful for establishing ...
How to Produce and Export a Multi-Channel Merged Image or Image Sequence for Publication
How to Produce and Export a Multi-Channel Merged Image or Image Sequence for Publication Volocity allows you to create high-quality merged images or image sequences suitable for publication. This can be done by capturing snapshots or using the "View ...
Avoiding Conflicts Between Volocity and Antivirus Software
Avoiding Conflicts Between Volocity and Antivirus Software Antivirus or SIEM software, including Windows Defender, may affect Volocity's performance, particularly due to real-time protection features. To prevent conflicts and ensure optimal ...
How to Change the Spatial Calibration of a Dataset in Volocity
How to Change the Spatial Calibration of a Dataset in Volocity Proper spatial calibration is essential for accurate quantitative analysis in Volocity. Each objective lens on the acquisition system should be calibrated using a stage graticule, which ...
How to Create a Measurement Item in Volocity
How to Create a Measurement Item in Volocity A Measurement Item allows you to save and analyze measurements performed on points, lines, or regions of interest in your image data. Follow these steps to create and store a Measurement Item in Volocity. ...
How to Enter an SMA Code in Volocity
How to Enter an SMA Code in Volocity To activate your Software Maintenance Agreement (SMA), follow these steps to enter your SMA code. On macOS: Start Volocity. Go to the Volocity menu and select Unlock Codes. Enter the code exactly as provided in ...
Exporting Charts for Use in Publications and Presentations
Exporting Charts for Use in Publications and Presentations You can export charts from Volocity for use in presentations or publications. This process involves capturing a snapshot of the chart, which creates a 2D image stored in the Volocity library. ...
How to Import a Measurement Protocol in Volocity
How to Import a Measurement Protocol in Volocity Importing a measurement protocol allows you to apply pre-configured analysis settings to your image data, saving time and ensuring consistent measurements across datasets. Follow these steps to import ...
Moving Items Between Libraries in Volocity
Moving Items Between Libraries in Volocity The most convenient way to move items between libraries in Volocity is by using the Library Clipping export format. This method allows you to export and import items seamlessly between libraries. Steps to ...
Understanding Software Autofocus Functionality
Software autofocus in Volocity is designed to find the optimal focal point by maximizing the signal-to-noise ratio (SNR) within the autofocus range. This means the algorithm identifies the point where the brightest point in the image divided by the ...
Generating Intensity-Modulated Display Images
Generating Intensity-Modulated Display Images An intensity modulated display image is created by combining information from a ratio image (e.g., a FRET image) and a modulator image to accurately represent both intensity and ratio data in a single ...
How to Derive Calibration Properties (µm/pixel) for Quantitative Analysis
How to Derive Calibration Properties (µm/pixel) for Quantitative Analysis If you are performing quantitative analysis on image data, it is most accurate to retrieve the calibration properties from the acquisition system or re-acquire the data after ...
How to Create a Debug Shortcut in Volocity
How to Create a Debug Shortcut in Volocity Creating a debug shortcut in Volocity can be very useful for troubleshooting issues, especially in the event of a crash. The debug log provides detailed insight into specific problems. Steps to Create a ...
How to Check the Time Remaining in Your Acquisition
How to Check the Time Remaining in Your Acquisition After starting a Volocity acquisition by pressing the Record button, you can track the time remaining in your acquisition. Steps to Check the Time Remaining: Locate the green box labeled Elapsed in ...
Lost Lightpaths After Moving Configuration Files to a Different Workstation – How to Reassign DCAM ID
Lost Lightpaths After Moving Configuration Files to a Different Workstation – How to Reassign DCAM ID When moving configuration files to a different workstation, the DCAM ID for connected cameras may need to be reassigned. Follow these steps on a ...
Why Do the Loop and Shuttle Options Work Under the Image Menu but Not Under the Movie Menu?
Why Do the Loop and Shuttle Options Work Under the Image Menu but Not Under the Movie Menu? The behavior of the Loop and Shuttle commands depends on whether you are working with an Image Sequence or a Movie in Volocity. Image Menu: The Loop and ...
The Size of the Video Preview Has Changed – How Do I Restore It?
The Size of the Video Preview Has Changed – How Do I Restore It? If you and your colleagues use the same user account, it’s possible that one of the following settings has been changed. Follow the steps below to identify and correct the issue. 1. ...
Tools Missing or Moved in the Toolbar
Tools Missing or Moved in the Toolbar In Volocity 5 or higher on macOS, the toolbar can be customized to include only the tools you need. If all users share the same macOS account, customizations might confuse some users. To move or restore a missing ...
What Is the Difference Between Z Step and µm/pixel (Z) in the Properties?
What Is the Difference Between Z Step and µm/pixel (Z) in the Properties? Z Step (µm): Refers to the physical step used to capture the original images. Changing this value does not affect how the data is represented in the image—it is purely for ...
What Is the Easiest Way to Capture Fluorescent and Brightfield Images Without Merging Them Manually?
What Is the Easiest Way to Capture Fluorescent and Brightfield Images Without Merging Them Manually? A more efficient method is to acquire two channels while using Manual Intervention in the Brightfield light path. This allows you to capture both ...
Why Are Focus Positions Not Stored When Creating XY Points?
Why Are Focus Positions Not Stored When Creating XY Points? If the focus position is not being stored when creating or reviewing XY points, it is likely because a Focus Device has not been set in the X-Y Stage preferences. Without a Focus Device ...
Why Can't I Change the Filter Turret Position with My Leica Microscope Using Volocity?
Why Can't I Change the Filter Turret Position with My Leica Microscope Using Volocity? If the filter turret does not move to the transmitted light position when requested in Volocity, this is likely due to the Contrast Mode setting on Leica ...
Why Can't I Make Changes to My Data After Importing It from a Portable Hard Drive or Another Workstation?
Why Can't I Make Changes to My Data After Importing It from a Portable Hard Drive or Another Workstation? If your dataset is still linked to a source file located outside the Volocity library (e.g., on a portable hard drive), you will not be able to ...
Why Do My Objects Appear Elongated in the Z Axis?
Why Do My Objects Appear Elongated in the Z Axis? Elongation in the Z axis is a common issue in optical microscopy and is often caused by a mismatch between the physical and optical step size between Z layers during imaging. Steps to Address Z-Axis ...
Why Is the Length of the 'Skeleton' Not Always Equal to the Skeletal Length Measurement in the Measurement Item?
Why Is the Length of the 'Skeleton' Not Always Equal to the Skeletal Length Measurement in the Measurement Item? The difference arises due to how Volocity calculates skeletal length and how the skeleton is visually represented. Definition of Skeletal ...
Why Is the "Calibrate Stage" Option Greyed Out Even Though My X-Y Stage Is Recognized?
Why Is the "Calibrate Stage" Option Greyed Out Even Though My X-Y Stage Is Recognized? The preferences for the X-Y stage are stored in two locations within Volocity: Device Preferences: Accessible via Edit > Preferences > Devices (or Volocity > ...
Configuring the Serial Port of the DMi8 for Volocity
Configuring the Serial Port of the DMi8 for Volocity When you install the Leica SDK (delivered with the DMi8 microscope), it includes a driver that allows the computer to connect to the microscope via USB. This driver sets up a "Virtual Com Port" ...
Volocity Supported File Formats
Volocity Supported File Formats File Formats for Import Volocity supports the following file formats for reading: BioRad PIC Windows BMP Volocity Library Clipping DeltaVision ICS/IDS (Image Cytometry Format) JPEG Leica LIF (generated by Leica ...
Volocity ILS & Windows Defender
Volocity ILS & Windows Defender Windows Defender is a preinstalled antivirus program in Windows 8.1 and Windows 10. Follow these steps to allow an application through Windows Defender: Step 1: Open Security and Maintenance Open the Windows Control ...