How to Produce and Export a Multi-Channel Merged Image or Image Sequence for Publication

Volocity allows you to create high-quality merged images or image sequences suitable for publication. This can be done by capturing snapshots or using the "View as..." export options. Below are detailed instructions for each method.

Method 1: Capture Snapshot (Single 2D Image)

This method captures a single 2D image from the current view and saves it in the millions of colors format. Snapshots can incorporate display options such as scales, color reference tables, or time overlays.

1. Open the Image Sequence:

   • In the Volocity Library, highlight the desired image sequence.
   • Double-click on the thumbnail to open the image sequence in its own window.

2. Select the Viewing Mode:

   • Switch to the Image Tab.
   • Use the dropdown menu to select the viewing mode (e.g., Extended Focus, XYZ, or other options).

3. Select the Correct Timepoint (if applicable):

   • For time series datasets, ensure the correct timepoint is selected.
   • Use the Time Navigation Bar below the view to switch between timepoints.
   • If the navigation bar is not visible, enable it by clicking on Image > Show Time Navigation.

4. Show or Hide Channels:

   • Use the Channel Controls on the right-hand side of the image window to display or hide specific channels.
   • If the channel controls are not visible, enable them via Image > Show Channels.

5. Edit the Display:

   • Add elements such as a scale bar, color reference table, or time display from the Image > Display menu.
   • Ensure the Z slice is correctly selected if viewing in XYZ, YZ, XZ, or XY mode.

6. Capture the Snapshot:

   • Go to Image > Capture Snapshot.
   • A new thumbnail for the snapshot will appear at the bottom of the library.
   • The snapshot incorporates all displayed options (e.g., scales, color tables, etc.) and is stored in the millions of colors format.

7. Export the Snapshot:

   • Highlight the snapshot in the library.
   • Go to File > Export.
   • Choose an export format suitable for publication:
     • Item as TIFF
     • Item as TIFF for Publication
   • Save the file to your desired location.

Method 2: Export Using the "View as..." Option (Merged Image or Movie)

This method is commonly used to quickly produce merged images or movies that appear exactly as they are shown in the Image View.

1. Open the Image Sequence:

   • In the Volocity Library, highlight the desired image sequence.
   • Double-click on the thumbnail to open it in its own window.

2. Select the Viewing Mode:

   • Switch to the Image Tab.
   • Use the dropdown menu to select the viewing mode (e.g., Extended Focus, XYZ, etc.).

3. Show or Hide Channels:

   • Use the Channel Controls on the right-hand side of the image window to display or hide specific channels.
   • If the channel controls are not visible, enable them via Image > Show Channels.

4. Edit the Display:

   • Add elements such as a scale bar, color reference table, or time display from the Image > Display menu.

5. Export the View:

   • Go to File > Export.
   • Select one of the View as... export options based on your requirements:
     • View as TIFF: Exports a single 2D image.
     • View as AVI Movie: Exports a time series dataset as a multi-channel merged movie.
     • Other formats, such as PNG or JPEG, are also available for single 2D images.

Key Considerations:

• For publication purposes, it is recommended to use the Item as TIFF for Publication option, as it provides high-quality output suitable for scientific figures.
• When exporting time series datasets as movies (e.g., AVI), ensure that all relevant channels and display options are configured before exporting.
• Always review the exported file to confirm it meets your publication or presentation needs.

By following these steps, you can produce and export merged images or sequences that are ready for use in scientific publications or professional presentations.