What Is the Easiest Way to Capture Fluorescent and Brightfield Images Without Merging Them Manually?
What Is the Easiest Way to Capture Fluorescent and Brightfield Images Without Merging Them Manually?
A more efficient method is to acquire two channels while using Manual Intervention in the Brightfield light path. This allows you to capture both fluorescent and brightfield images without acquiring and merging them separately.
Steps to Enable Manual Intervention:
- Double-click the Light Path Manager button.
- In the Light Path Properties dialog, check the box for Requires Manual Intervention if the light path requires manual adjustments.
- When this light path is called during acquisition, a message will prompt you to make the necessary manual changes.
This process streamlines the acquisition of both fluorescent and brightfield images in a single workflow.